tRNA nucleotidyltransferase
| tRNA nucleotidyltransferase | |||||||
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| Identifiers | |||||||
| EC number | 2.7.7.56 | ||||||
| CAS number | 116412-36-3 | ||||||
| Databases | |||||||
| IntEnz | IntEnz view | ||||||
| BRENDA | BRENDA entry | ||||||
| ExPASy | NiceZyme view | ||||||
| KEGG | KEGG entry | ||||||
| MetaCyc | metabolic pathway | ||||||
| PRIAM | profile | ||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||
| Gene Ontology | AmiGO / EGO | ||||||
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In enzymology, a tRNA nucleotidyltransferase (EC 2.7.7.56) is an enzyme that catalyzes the chemical reaction
- tRNAn+1 + phosphate
tRNAn + a nucleoside diphosphate
Thus, the two substrates of this enzyme are tRNA(n+1) and phosphate, whereas its two products are tRNA(n) and nucleoside diphosphate.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is tRNA:phosphate nucleotidyltransferase. Other names in common use include phosphate-dependent exonuclease, RNase PH, and ribonuclease PH.
Structural studies
As of late 2007, 9 structures have been solved for this class of enzymes, with PDB accession codes 1OYP, 1OYR, 1OYS, 1R6L, 1R6M, 1UDN, 1UDO, 1UDQ, and 1UDS.
References
- Cudny H, Deutscher MP (1988). "3' processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement". J. Biol. Chem. 263 (3): 1518–23. PMID 3275667.
- Deutscher MP, Marshall GT, Cudny H (1988). "RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase". Proc. Natl. Acad. Sci. U. S. A. 85 (13): 4710–4. doi:10.1073/pnas.85.13.4710. PMC 280505. PMID 2455297. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=280505.
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